Conformational Mechanism for the Stability of Microtubule-Kinetochore Attachments
نویسندگان
چکیده
منابع مشابه
Conformational mechanism for the stability of microtubule-kinetochore attachments.
Regulating the stability of microtubule (MT)-kinetochore attachments is fundamental to avoiding mitotic errors and ensuring proper chromosome segregation during cell division. Although biochemical factors involved in this process have been identified, their mechanics still need to be better understood. Here we introduce and simulate a mechanical model of MT-kinetochore interactions in which the...
متن کاملCLIP-170 facilitates the formation of kinetochore-microtubule attachments.
CLIP-170 is a microtubule 'plus end tracking' protein involved in several microtubule-dependent processes in interphase. At the onset of mitosis, CLIP-170 localizes to kinetochores, but at metaphase, it is no longer detectable at kinetochores. Although RNA interference (RNAi) experiments have suggested an essential role for CLIP-170 during mitosis, the molecular function of CLIP-170 in mitosis ...
متن کاملTheory of strength and stability of kinetochore-microtubule attachments: collective effects of dynamic load-sharing
Application of pulling force, under force-clamp conditions, to kinetochore-microtubule attachments in-vitro revealed a catch-bond-like behavior. In an earlier paper (Sharma et al. Phys. Biol. (2014) the physical origin of this apparently counter-intuitive phenomenon was traced to the nature of the force-dependence of the (de-)polymerization kinetics of the microtubules. In this brief communicat...
متن کاملMechanisms of force generation by end-on kinetochore-microtubule attachments.
Generation of motile force is one of the main functions of the eukaryotic kinetochore during cell division. In recent years, the KMN network of proteins (Ndc80 complex, Mis12 complex, and KNL-1 complex) has emerged as a highly conserved core microtubule-binding complex at the kinetochore. It plays a major role in coupling force generation to microtubule plus-end polymerization and depolymerizat...
متن کاملRegulation of kinetochore-microtubule attachments by Aurora B kinase.
Accurate segregation of chromosomes in mitosis requires that spindle microtubules attach sister kinetochores to opposite poles of the mitotic spindle (biorientation). To achieve biorientation of all chromosomes, incorrect attachments are selectively destabilized, providing a fresh opportunity to biorient, whereas correct attachments are stabilized. Tension across the centromere may be the signa...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2014
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2014.06.004